Technology Transfer Session
Oral Presentations – August 13, 2024 – Room: “Anatomisch theater“
Time
Title
Speaker
14:00-14:15
Jason Lombard (United States)
14:15-14:30
Ann-Kristin Nyman (Sweden)
14:30-14:45
Pablo Munoz-Boettcher (Chile)
14:45-15:00
Joaquín Barca (Uruguay)
15:00-15:15
Jose Augusto Ferronatto
(Brazil)
15:15-15:30
Martina Penati
(Italy)
15:30-15:45
Mariana Fonseca (Canada)
15:45-16:00
Jacob Diderich (Belgium)
16:00-16:15
Olivier Salat
(France)
16:15-16:30
Ulrike Sorge
(Germany)
Poster Presentations – August 12, 13 & 14 2024
Poster
Title
First author
1
The antimicrobial activity of rifaximin against bovine mastitis Staphylococcus aureus
Florencia Aliverti (Argentinia)
2
Melaleuca armillaris essential oil as teat dip to control subclinical mastitis in dairy cows
Florencia Aliverti (Argentinia)
3
Perceptions of U.S. dairy producers regarding milk quality and antimicrobial usage during dry-off
Tana Jo Almand (United States)
4
Milk yield relative to day of early lactation clinical mastitis in pegbovigrastim treated cows
Joaquín Barca
(Chile)
5
Case Study: Genetic characterization of methicillin and lincosamide resistant Mammaliicoccus fleurettii isolated from cattle and humans on a Vermont organic dairy farm
John Barlow
(United States)
6
Effect of Smart DCT algorithm on the antimicrobial use at dry-off compared to five other algorithms
Silvia Beschi
(Italy)
7
IDEXX real PCR MILQ-ID DNA system: New solution for mastitis testing
Loïc Commun (Sweden)
8
Comparison of two commercial rapid tests for bacteriological culture of milk samples from non-severe clinical mastitis cases
Lien Creytens (Belgium)
9
Evaluation of an AI-based automatic image classifier for analysis of bacterial growth on a mastitis multiple-agar plate
Josef Dahlberg (Sweden)
10
Revised in vitro simulation model to evaluate germicidal activity in alternatives for teat disinfection products
Sara De Groote (Belgium)
11
Development of a rapid semi-quantitative immunochromatographic test for on-farm subclinical mastitis detection
Pauline Delhez (Belgium)
12
Can we use on-farm tests for diagnosing intramammary infection at dry-off in dairy cows?
José Denis-Robichaud (Canada)
13
Case report: Optimization of spray performance of teat disinfectants in automatic milking systems
Madelena De Ro (Belgium)
14
Twelve-month follow-up of the udder microbiota of Norwegian red dairy cow
Vinícius Duarte (Brazil)
15
Dairy farmers' perception of udder health consulting and advice from the herd veterinarian
Michael Farre (Denmark)
16
Employee management as risk factor associated with bulk tank somatic cell count and new infection risk
Michael Farre (Denmark)
17
Exploring bovine milk microbiota using high-throughput sequencing: insights from a robust analysis
Jose Augusto Ferronatto
(Brazil)
18
Unveiling bovine colostrum microbiota through high-throughput sequencing: insights from a rigorous analysis
Jose Augusto Ferronatto
(Brazil)
19
Evidence of bovine mammary gland sterility in nulliparous dairy heifers: Preliminary results
Jose Augusto Ferronatto
(Brazil)
20
Case Report: The influence of waste milk feeding on antimicrobial resistance in dairy calves in Austria: a pilot study under field conditions
Clair Firth (Austria)
21
Performance of somatic cell score and differential somatic cell count in identifying quarters with subclinical mastitis using Gaussian finite mixture model
Mariana Fonseca (Canada)
22
Methicillin-resistant staphylococci and mammaliicocci in the bulk tank milk of dairy herds from a high-density livestock farming area of northern Italy
Sara Fusar Poli (Italy)
23
Antimicrobial resistance In bovine Staphylococcus aureus strains isolated in northern Italy
Alessandra Gazzola (Italy)
24
Antimicrobial resistance patterns in predominant bacterial bovine mastitis pathogens in Slovenia
Majda Golob (Slovenia)
25
In vitro antimicrobial susceptibility of Staphylococcus spp. isolated from milk of goats in intensive and extensive production systems
Lihuel Gortari Castillo (Argentina)
26
Colostrum collection using robotic milking systems: Impact on microbiological quality
Geert Hoflack (Belgium)
27
Colostrum collection using robotic milking systems: Impact on immunoglobulin concentration
Geert Hoflack (Belgium)
28
Predicting mastitis in dairy cows milked by robot systems
Floris Herrema (The Netherlands)
29
A non-antibiotic approach to improving udder health in Irish dairy cows
Nicole Keohane McCarthy (Ireland)
30
Effects of feeding heat-killed Lactobacillus plantarum L-137 on perinatal immunity and milk properties during the dry period in cows
Yoshio Kiku (Japan)
31
Antimicrobial resistance patterns of Staphylococcus epidermidis isolated from bovine mastitis in Denmark
Desiree Kløve (Denmark)
32
Microbiological background of haemolactia in Holstein-Friesen dairy cows
Peter Kovacs (Hungary)
33
Rapid mastitis diagnostics using PCR in the vet practice
Christophe Kunas (Switzerland)
34
Increased serum prostaglandin E2 concentrations during inflammation correlate with plasma triacylglycerides Δ9 desaturase index and milk fatty acid chain length
Chrissy Lalonde (United States)
35
Penicillin susceptibility of Streptococcus uberis in France : Reliability of gradient-diffusion method and comparison of MIC values between strains from recent or chronic infections
Guillaume Lequeux (France)
36
Highly Pathogenic Avian Influenza Virus in dairy cattle in the U.S.: A case report
Jason Lombard (United States)
37
Feasibility of on-farm detection of mastitis pathogens within four hours
Eimear Looby (Ireland)
38
Determination of antimicrobial resistance and detection of resistance genes in Staphylococcus aureus causing bovine mastitis in Argentina
Mariana Lucas (Argentina)
39
In vitro antimicrobial resistance of Streptococcus uberis isolated from bovine mastitis in Argentina
Mariana Lucas (Argentina)
40
Improving udder health together
Åsa Lundberg (Sweden)
41
The ‘Cell Count Solutions’ training programme: An evaluation of a blended-learning multidisciplinary training programme for mastitis control consults
Michelle McGrath (Ireland)
42
Visualization of Mycoplasma bovis biofilm formation from Chilean bovine mastitis isolates using Scanning Electron Microsopy (SEM)
Armin Mella
(Chile)
43
Characterization of Mycoplasma bovis strains isolated from bovine mastitis outbreaks in three dairy farms in southern Chile
Armin Mella
(Chile)
44
The prevalence of variant GTRI in Staphylococcus aureus isolates from alpine grazing system in the Aosta Valley
Valentina Monistero (Italy)
45
Investigation of in-situ generated performic acid for teat disinfection with favorable biocidal and chemical residue profile
Joseph Morelli
(United States)
Poster
Title
First author
46
Milking behavior dynamics within the week before clinical mastitis diagnosis according to pathogen category in dairy cows milked by an automated milking system
Pablo Munoz-Boettcher
(Uruguay)
47
A case report of 481 lactococci isolates from Swedish dairy cows with intramammary infections
Ann-Kristin Nyman (Sweden)
48
Virulence factors of bovine-associated Staphylococcus aureus: clonal, origin and herd diversity
Ana Oliviera
(Brazil)
49
Genotypic and phenotypic characterization of biofilm in bovine-associated Staphylococcus aureus
Ana Oliviera
(Brazil)
50
Case report on the effect of bimodal milk flow on mastitis infection with optimized pre-treatment time in automatic milking systems
Ruben Otten
(The Netherlands)
51
Species identification by MALDI-TOF MS of non-aureus staphylococci and mammaliicocci (NASM) isolated from cow milk: Revealing the prevalence of Staphylococcus borealis and Staphylococcus rostri
Martina Penati
(Italy)
52
In vitro impact of bovine-associated non-aureus staphylococci (NAS) isolates against Staphylococcus aureus and Streptococcus uberis using mammary epithelial cell lines.
Gregory Rota (Belgium)
53
Etiology of non severe mastitis in french dairy herds
Olivier Salat
(France)
54
Detection of Mollicutes and Mycoplasma bovis in bulk tanks milk in Brazil
Anelise Salina
(Brazil)
55
Validation of a Streptococcus uberis mastitis model in late lactation
Christian Scherpenzeel
(The Netherlands)
56
Trends in bulk milk somatic cell Count in The Netherlands from 2018 - 2023
Christian Scherpenzeel
(The Netherlands)
57
Validation of an Escherichia coli Mastitis Model
Christian Scherpenzeel
(The Netherlands)
58
Verifying hygiene protocol success on dairy farms with problematic DHI results by ATP testing: A case report
Bjoern Schroeder (Switzerland)
59
A case report of 613 yeast isolates from dairy cows with intramammary infections
Dinah Seligsohn
60
Effect of dry-off procedure and dry-off treatment on somatic cell count
Pablo Silva Bolona (Ireland)
61
Treating mastitis with milk from healthy quarters – A pilot study
Ulrike Sorge (Germany)
62
High somatic cell Count at first DHI test day associated with lower lifetime milk production in Simmental dairy cows
Ulrike Sorge (Germany)
63
The effect of pegbovigrastim at dry-off on oxidative stress during early mammary gland involution
Jamie Strickland (United States)
64
Trends in bulk tank milk culture results in the Netherlands
Sabine Tijs
(The Netherlands)
65
Expression and secretion of galectin 7 a biomarker in cow milk and its possible role in host defense
Mulumebet (Millie) Worku
(United States)
67
Evaluation of selective dry cow therapy in Czech dairy herd – Three years’ experience – Case report
Josef Prášek
(Czech Republic)
68
Stability and lytic activity assessment in milk of bacteriophages targeting Escherichia coli causing bovine mastitis
Jacob Diderich (Belgium)
69
Whole-genome sequencing reveals genetic differences of Streptococcus uberis isolated from cows with transient or persistent intramammary infection.
Anyaphat Srithanasuwan (Thailand)
70
Manure pro conditioner improves bedding quality and bacterial composition with potential beneficial impacts for dairy cow’s health
Bastien Frayssinet
(France)
Scientific committee
Highly pathogenic avian influenza (HPAI) H5N1 virus has been reported in wild birds and poultry in the U.S. and many countries around the world. On March 25, 2024, HPAI H5N1 was detected in two dairy herds in the U.S. About 10-20 percent of lactating cows showed clinical signs in affected herds. Anecdotal reports were that older cows and those in mid to late lactation were most often affected with a dramatic decline in milk production. Cases generally peak about 5-7 days after the first clinical signs are detected. Most cows recover with supportive care, while other cows die or are removed from the herd. A large amount of virus is found in the milk from clinically affected cows. This is an emerging disease event and there are many unknowns regarding the disease. Many agencies are working to better understand this disease in dairy cattle.
A Case Report of 481 Lactococci Isolates from Swedish Dairy Cows with Intramammary Infections. Nyman. In the present study the distribution of different lactococci species in bovine milk samples were investigated as well as cow and herd characteristics. Most lactococci species were Lactococcus (L.) lactis (74% of the samples) or L. garvieae/formosensis/petauri (24% of the samples) and were mainly from cases of subclinical mastitsi. Few differences in cow and herd characteristics were found, but it was concluded that few cows spontaneous cured as the somatic cell count remained high several months after a case of lactococci IMI. This finding can be helpful for the farmer and/or veterinarian to make decisions on treatments or other measures for the cow.
Key words: subclinical mastitis, clinical mastitis, cure, milk production, parity, seasonality
The objective was to describe and compare the dynamics of specific milking behaviors, including milking duration, milking interval, incomplete milkings, and kick-offs in cows developing clinical mastitis by etiological agent category and lactation period.
Dairy cows with clinical mastitis caused by gram-negative bacteria spent more time in the milking unit and had longer milking intervals than other pathogen categories. Lastly, gram-negative bacteria were associated to a higher probability of incomplete milking than gram-positive bacteria and healthy animals. This research seeks to provide more information about the effect of different groups of pathogens on the milking behavior of cows in an automated milking system.
Keywords: milking behavior, automated milking system, clinical mastitis.
Milk yield losses associated to clinical mastitis (CM) represent one of the main costs of the disease. It has been proposed that immune stimulation therapies could mitigate this problem. In this randomized trial, we explore the reduction in milk yield associated with CM during the first 30 days in milk (DIM) in cows that were treated with pegbovigrastim in comparison to untreated controls. Cows with CM during the first 30 DIM suffered a milk drop from the day of diagnosis up until 305 d after diagnosis (approximately 3 kg/d until 42 d and 1.7 kg/d afterwards). In PEG treated cows, the milk drop associated with CM was almost 60% smaller, as a milk yield compensation in PEG treated cows was detected from 15 d to 305 d relative to the diagnosis. Our data suggest that PEG could be a valuable tool to limit the milk yield drop associated with CM.
Keywords: pegbovigrastim, clinical mastitis, transition, milk yield
There is no consensus on the presence of viable microbial communities in the bovine mammary gland or when, if any, they are established. While some studies support that contaminating microbial DNA from low microbial biomass sites is the only source detected, aside from DNA amplification of non-viable bacteria, others suggest an endogenous entero-mammary pathway in dairy cows. Such divergence over the fundamental aspect of bovine mammary gland biology poses an obstacle to scientific progress. Therefore, we aimed to determine whether the presence of viable bacterial DNA in the mammary gland from nulliparous non-pregnant heifers supports the existence of a microbiome during their development. A high similarity of ASVs in control and tissue samples was found, indicating that the microbial signals detected are likely the result of contamination during tissue sampling, DNA extraction, and sequencing. Thus, our study supports the concept of sterility in the bovine mammary gland of young animals.
This study investigates the identification of non-aureus staphylococci and mammaliicocci (NASM) in cow milk using MALDI-TOF MS, focusing on the prevalence of Staphylococcus borealis and Staphylococcus rostri. The research included 23,463 milk samples from over 100 dairy farms in Northern Italy, collected between May 2021 and November 2023. NASM species were identified using the Bruker Biotyper System Compass® Library, with updates to include spectra for the newly classified S. borealis and S. rostri. Results showed S. chromogenes as the most prevalent NASM in both clinical and subclinical mastitis, with S. borealis and M. sciuri also being significant. According to the results, the prevalence of S. borealis and S. rostri was previously underestimated. Accurate identification of NASM is crucial for understanding their impact on udder health, potentially informing better farm management practices to improve milk quality.
Mastitis is the most common disease affecting dairy cows in North America, entailing significant economic losses for the dairy industry. The application of Gaussian finite mixture model introduces novel perspectives into the detection of subclinical intramammary infections (IMIs) using somatic cell score (SCS) and differential somatic cell count (DSCC). Our findings suggest that SCS and DSCC could be used for identifying quarters with IMI, and that SCS seemed to surpass DSCC for predicting subclinical mastitis probability.
Bovine mastitis is a major production disease in dairy cattle and complementary treatments to antimicrobials are urgently needed to fight antibiotic resistances. Intramammary phage therapy is a promising approach but characterizing isolated phages in milk is a crucial initial step. This study aimed to compare the stability and lytic activity of 10 newly isolated phages targeting E. coli in milk. Our study indicates that milk components are not an obstacle to phage therapy in the context of E. coli mastitis. However, bacterial regrowth during the in vitro efficacy assessment suggests the presence of resistances that could be bypassed with the use of phage cocktails.
The aims of this study were to evaluate performances of gradient-diffusion method to determine penicillin MIC in S.uberis strains, describe the repartition of penicillin MIC values determined by this gradient-diffusion method in recent S.uberis French strains and to compare penicillin MIC values of S.uberis strains isolated from recent mastitis with those obtained from strains isolated from chronic mastitis. Prevalence of reduced susceptibility to penicillin remains low (<1%) and no resistance was detected. No difference in penicillin MIC values between strains from chronic or recent infections was observed. Our results highlight that gradient-diffusion is a reliable method to determine in routine penicillin MIC in S.uberis, a key information for dairy practitioners to (i) survey reduced susceptibility to penicillin (ii) evaluate the probability of cure rate after antimicrobial therapy (iii) determine the most appropriate antimicrobial therapeutic scheme to treat S. uberis infections.
Keywords: Streptococcus uberis, penicillin, gradient-diffusion MIC test
Effective alternatives to antibiotic treatment of mastitis are needed. This study investigated whether application of milk from a healthy quarter could aid cows in restoring their milk microbiome and achieving bacteriological cure of a mastitic quarter. Cows were treated one or twice with milk from one of their healthy quarters and the cure compared to those of untreated cows. In the end there were no adverse effects by the treatment. However, there were also no effects on bacteriological cure, a reduction in somatic cell counts or the milk microbiome during the three week study period.
Keywords: mastitis, microbiome, milk, treatment